Mushroom Growing Substrate: Complete Guide (2024)

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Growing mushrooms starts with selecting the right mushroom growing substrate for your grow. The mushroom growing substrate is another word for the mushroom growing medium. This is what the mushroom mycelium grows through and it provides nutrition to the mushroom mycelium and subsequent fruiting mushrooms.

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You can think of a mushroom growing substrate just like soil for plants – some plants require special soil or enriched soil, while others don’t. In this guide, we will break down the different substrates that common growing mushrooms prefer.

Choosing a Mushroom Growing Substrate

The key part to understand is that every mushroom tends to grow best in certain types of substrates. For example, a King Oyster mushroom may grow best in hardwood sawdust, while a White Cap mushroom may prefer Manure.

As stated above, the nutrition of each substrate is what the mushrooms depend on to grow. This can be protein content, sugar content, potassium, fats, and nitrogen.

Another key component of the mushroom growing substrate is the water content. Many substrates require water as an added ingredient, but the amount of water is important. This can sometimes be 1/4 water as the volume of the ingredients, but it highly depends on the mushroom chosen.

You may have learned from other sources that sterilization of the substrate is important, and this cannot be understated. Using a sterilized growing medium is extremely important, otherwise you risk mold overtaking your substrate and ruining the mycelium. Always use safe and effective methods to sterilize your mushroom growing substrate, such as a pressure cooker.

Learn how to sterilize substrates in our guide here.

Mushroom Growing Substrate Examples

King Oyster Mushroom Substrate

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King Oyster mushrooms grow best on cellulose-rich mediums. This is usually going to be sawdust, straw, or a mix of the 2. Our best recommendation for king oyster substrate is the following:

  • Blend of hardwood sawdust (Amazon)
  • Supplement of 5-10% of wheat bran (Amazon)
  • 60-70% moisture content
  • a ph level of 6.5 after sterilization.
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These conditions and ingredients will allow the king oyster mushrooms to grow best. Other mediums can prove successful, but this blend is known to be highly effective.
Always take into consideration the other aspects of a well-controlled grow such as temperature of the grow room and lighting. While we can’t cover all of that detail in this guide, we have plenty of guides on that information on our site here.

Shiitake Mushroom Substrate

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Shiitake mushrooms grow best on wood-based mediums. Much like the king oyster mushroom, they prefer hardwood sawdust. Other mediums will work, but Shiitake mushrooms are more picky than Oyster mushrooms and it’s best to choose sawdust or other forms of ground wood. Here are the ingredients for Shiitake substrates:

  • Blend of hardwood sawdust (Amazon)
  • Supplement of 0-5% of wheat bran (Amazon)
  • 55% moisture content
  • a ph level of 6.5 after sterilization.

Portabello Mushroom Substrate

Portabello mushrooms prefer an alternative substrate of manure-based compost (Amazon). This will usually be a compost that is blended with manure. This should be easy to find, as many types of compost already come mixed with manure.

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You will still perform the normal steps of sterilization, and layer your manure-based compost into a wide cubic bin. Then, you’ll pour in some of your portabello spores and then leave the substrate to grow. This can be done indoors or outdoors, but given that compost/manure can be a bit messier than other substrates, outside is best.

White Button Mushroom Substrate

White Button mushrooms also prefer manure-based compost (Amazon) as their preferred growing medium. This will be a similar process to the above portabello guide.

An easy trick for growing white button mushrooms is to combine a 50/50 blend of manure and compost and blend it up. Now, with this substrate prepared, you can be assured you’re using the best substrate for white button mushrooms.

Conclusion

We listed a few of the most popular mushroom’s favorite growing mediums. These are some of the mushrooms that are easiest to grow at home. In the entire kingdom of mushrooms, there are so many mushrooms that require such special growing circ*mstances that you simply can’t grow them at home. This includes mushrooms that require a fresh forest burn to grow, or mushrooms that grow on dying trees.

Because of this, choosing easy-to-grow mushrooms is important, especially as a beginning grower. Choose a simple mushroom with an simple substrate and get started.

To really drive the point home, we highly recommend you take the sterilization of your growing substrate very seriously. Much of your hard work can go to waste if you don’t properly sterilize your mushroom growing medium with a pressure sterilization method.

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Mushroom Growing Substrate: Complete Guide (2024)

FAQs

How much mushroom substrate do I need? ›

The amount of bulk substrate and spawn you use will be dependent on the size of your monotub. You want to have 3-5 inches of substrate. One 3lb injection port bag should readily inoculate one or two moderate sized tubs.

What are the steps in mushroom substrate? ›

Contents
  1. STEP 1: Growing out a chosen mushroom culture on an agar filled petri dish.
  2. STEP 2: Transferring the mushroom culture (known as mycelium) onto sterilized grain.
  3. STEP 3: Expanding the grain jar exponentially to create grain spawn.
  4. STEP 4: Spawning the grain into a suitable substrate and fruiting container.

How much mycelium is enough? ›

The common standard of spawning is 8-10 liters, or 5-6 kilograms of mycelium per ton of phase 2 compost.

What is the best ratio for mushroom substrate? ›

The recommended spawn to substrate ratio for growing mushrooms, such as cubensis, b plus mushrooms, or oyster mushrooms, is around 1:2 to 1:4. This means that for the psilocybe cubensis substrate recipe, one part of grain spawn should be used for every two to four parts of mushroom substrate.

How do I calculate how much substrate I need? ›

here is how to work out how many litres of substrate you need for a given tank size. And also how to calculate your tank volume in total if you need to. the formula is: (length x width x depth) / 1000 = litres.

How much substrate is enough? ›

Substrate: How Much Do I Need?
Aquarium Size* (gallons)#s of Substrate
1010 - 20
20L/29/3729 - 58
40L/5555 - 110
120120 - 240
1 more row

What is the easiest mushroom substrate? ›

Pretty much every mushroom growing resource I could find says that oyster mushrooms are the easiest variety for first time-growers, as they grow fast and can easily thrive in substrates made of things like coffee grounds and straw, making them relatively low maintenance.

What is the best substrate for mycelium growth? ›

These are high in carbon, which is your mycelium's primary food supply. Straw or hardwood sawdust are common substrates for growing mushrooms, but there are a variety of other good options to consider.

How long does it take for mushroom substrate to colonize? ›

Colonization occurs after inoculation. This is when the mycelium will start to take over the grain that was inoculated with spores. This period usually lasts between 3 to 6 weeks depending on strain and environmental conditions. Ideal conditions for this phase are in a dark place and temperature between 72 F – 80 F.

What is the best grain for Cubensis? ›

What Is the Best Grain for Mushroom Spawn?
  • Rye Grain. Rye grain has fantastic water retention capabilities. ...
  • Wheat Berries. Wheat berries are also a great choice. ...
  • Popcorn Spawn. ...
  • Wild Bird Seed. ...
  • Milo-Grain Sorghum. ...
  • The Power of Millet. ...
  • Working with Rye.
Feb 9, 2023

What does healthy mycelium look like? ›

Spend time getting to know what your mycelium is supposed to look like so you can better identify unhealthy or contaminated patches. While mycelium is typically white and filamentous, contamination generally takes the form of green, blue, gray, or black patches or discolorations in your substrate.

What makes mycelium grow faster? ›

Light: While mycelium doesn't require light to grow, a small amount can help stimulate growth. 5. Nutrition: Using a nutrient-rich substrate can also enhance growth. Remember, it's important to maintain sterile conditions to prevent contamination.

Can mushroom substrate be too wet? ›

If you have insufficient moisture, as stated earlier, your mycelium growth will be stunted. However, if you have too much moisture, your mycelium will also be stunted, as it will suffocate. With too much moisture, your substrate will also have a higher risk of contamination from other organisms.

Can Cubensis grow on straw? ›

On the other hand, growing psilocybe cubensis on straw can also yield good results, although it may require a bit more attention to detail regarding environmental conditions.

How much coffee grounds to add to mushroom substrate? ›

Coffee grounds should comprise 77% of your substrate's total weight. Pasteurized straw or sawdust equal to 15% of the total substrate weight or 20% of the weight of the coffee grounds. Oyster or sh*take mushroom spawn equal to 8% of the total substrate weight or 10% of the weight of coffee grounds.

Is 1 inch of substrate enough for plants? ›

The substrate should be at least 2 inches thick. This will give the plants plenty of room to root and physically push the plants into - but if you have a bit more, it will be easier. You can also add root tabs to the substrate if you wanted to give it an extra boost.

Is 1 inch of substrate enough? ›

Concerning the desired depth, it can vary from 1 to 4 inches (2.5 to 10 cm) depending on the aquarium's scale and the plants' needs. If you are planning on a significant slope of substrate, you will have to visualize where the midpoint of this slope will be and make that point an estimated depth.

How much spawn for 5 lbs of substrate? ›

We recommended 1 lb of colonized grain spawn for every 5 lbs of manure or hardwood sterile substrate. We also recommend using 2.5-5ccs per 3 lb injection port bag.

How much substrate per mushroom bag? ›

We feel the best ratio is 1:2 spawn to sub. A 5 lb bag can be spawned into two individual 16 qt (13″x7″x8″) bins with a single 2.5 qt (2.5 lbs) bag of spawn. A 5 lb bag can be spawned into a 54 qt Sterelite tub (22.5″ x 16″ x 12.75″) with 2.5 – 5 qts (2.5 – 5 lbs) of spawn.

References

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